cells for protein overexpression Search Results


plasma membrane vesicles sf9 cells overexpressing breast cancer resistance protein (bcrp)  (Solvo Biotechnology)
90
Solvo Biotechnology plasma membrane vesicles sf9 cells overexpressing breast cancer resistance protein (bcrp)
Screening for ATP-dependent uptake of E2-17β-G and 25OHD3-G in plasma membrane vesicles overexpressing efflux transporters. (A) E2-17β-G, a positive control substrate, was incubated at 50 μM with plasma membrane vesicles overexpressing MRP2, MRP3, BCRP, and MRP4 as well as their corresponding mock control membranes at 37°C for 5 minutes. (B) 25OHD3-G was incubated at 2 μM with plasma membrane vesicles overexpressing MRP2, MRP3, BCRP, and MRP4 as well as their corresponding mock control membranes at 37°C for 5 minutes. Data shown are means ± S.D. of triplicate determinations in a single experiment. Differences between the ATP and AMP groups and between the transporter-overexpression and the mock <t>[Sf9</t> or human embryonic kidney (HEK)] groups for each transporter were analyzed with Student’s t test; differences with P values of <0.05 were considered statistically significant. *P < 0.05; **P < 0.01; ***P < 0.001.
Plasma Membrane Vesicles Sf9 Cells Overexpressing Breast Cancer Resistance Protein (Bcrp), supplied by Solvo Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasma membrane vesicles sf9 cells overexpressing breast cancer resistance protein (bcrp)/product/Solvo Biotechnology
Average 90 stars, based on 1 article reviews
plasma membrane vesicles sf9 cells overexpressing breast cancer resistance protein (bcrp) - by Bioz Stars, 2026-03
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hct116 cells with stable overexpression of firefly luciferase (fluc) and enhanced green fluorescent protein (egfp)  (Imanis Life Sciences LLC)
90
Imanis Life Sciences LLC hct116 cells with stable overexpression of firefly luciferase (fluc) and enhanced green fluorescent protein (egfp)
Screening for ATP-dependent uptake of E2-17β-G and 25OHD3-G in plasma membrane vesicles overexpressing efflux transporters. (A) E2-17β-G, a positive control substrate, was incubated at 50 μM with plasma membrane vesicles overexpressing MRP2, MRP3, BCRP, and MRP4 as well as their corresponding mock control membranes at 37°C for 5 minutes. (B) 25OHD3-G was incubated at 2 μM with plasma membrane vesicles overexpressing MRP2, MRP3, BCRP, and MRP4 as well as their corresponding mock control membranes at 37°C for 5 minutes. Data shown are means ± S.D. of triplicate determinations in a single experiment. Differences between the ATP and AMP groups and between the transporter-overexpression and the mock <t>[Sf9</t> or human embryonic kidney (HEK)] groups for each transporter were analyzed with Student’s t test; differences with P values of <0.05 were considered statistically significant. *P < 0.05; **P < 0.01; ***P < 0.001.
Hct116 Cells With Stable Overexpression Of Firefly Luciferase (Fluc) And Enhanced Green Fluorescent Protein (Egfp), supplied by Imanis Life Sciences LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hct116 cells with stable overexpression of firefly luciferase (fluc) and enhanced green fluorescent protein (egfp)/product/Imanis Life Sciences LLC
Average 90 stars, based on 1 article reviews
hct116 cells with stable overexpression of firefly luciferase (fluc) and enhanced green fluorescent protein (egfp) - by Bioz Stars, 2026-03
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l6 cell line overexpressing glut4 with a myc protein tag (glut4-myc)  (AstraZeneca ltd)
90
AstraZeneca ltd l6 cell line overexpressing glut4 with a myc protein tag (glut4-myc)
Screening for ATP-dependent uptake of E2-17β-G and 25OHD3-G in plasma membrane vesicles overexpressing efflux transporters. (A) E2-17β-G, a positive control substrate, was incubated at 50 μM with plasma membrane vesicles overexpressing MRP2, MRP3, BCRP, and MRP4 as well as their corresponding mock control membranes at 37°C for 5 minutes. (B) 25OHD3-G was incubated at 2 μM with plasma membrane vesicles overexpressing MRP2, MRP3, BCRP, and MRP4 as well as their corresponding mock control membranes at 37°C for 5 minutes. Data shown are means ± S.D. of triplicate determinations in a single experiment. Differences between the ATP and AMP groups and between the transporter-overexpression and the mock <t>[Sf9</t> or human embryonic kidney (HEK)] groups for each transporter were analyzed with Student’s t test; differences with P values of <0.05 were considered statistically significant. *P < 0.05; **P < 0.01; ***P < 0.001.
L6 Cell Line Overexpressing Glut4 With A Myc Protein Tag (Glut4 Myc), supplied by AstraZeneca ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/l6 cell line overexpressing glut4 with a myc protein tag (glut4-myc)/product/AstraZeneca ltd
Average 90 stars, based on 1 article reviews
l6 cell line overexpressing glut4 with a myc protein tag (glut4-myc) - by Bioz Stars, 2026-03
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human glioblastoma u251-mg cells stably overexpressing the wild-type human amyloid precursor protein (u251-app751)  (Korth Kristalle)
90
Korth Kristalle human glioblastoma u251-mg cells stably overexpressing the wild-type human amyloid precursor protein (u251-app751)
Screening for ATP-dependent uptake of E2-17β-G and 25OHD3-G in plasma membrane vesicles overexpressing efflux transporters. (A) E2-17β-G, a positive control substrate, was incubated at 50 μM with plasma membrane vesicles overexpressing MRP2, MRP3, BCRP, and MRP4 as well as their corresponding mock control membranes at 37°C for 5 minutes. (B) 25OHD3-G was incubated at 2 μM with plasma membrane vesicles overexpressing MRP2, MRP3, BCRP, and MRP4 as well as their corresponding mock control membranes at 37°C for 5 minutes. Data shown are means ± S.D. of triplicate determinations in a single experiment. Differences between the ATP and AMP groups and between the transporter-overexpression and the mock <t>[Sf9</t> or human embryonic kidney (HEK)] groups for each transporter were analyzed with Student’s t test; differences with P values of <0.05 were considered statistically significant. *P < 0.05; **P < 0.01; ***P < 0.001.
Human Glioblastoma U251 Mg Cells Stably Overexpressing The Wild Type Human Amyloid Precursor Protein (U251 App751), supplied by Korth Kristalle, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human glioblastoma u251-mg cells stably overexpressing the wild-type human amyloid precursor protein (u251-app751)/product/Korth Kristalle
Average 90 stars, based on 1 article reviews
human glioblastoma u251-mg cells stably overexpressing the wild-type human amyloid precursor protein (u251-app751) - by Bioz Stars, 2026-03
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altering cell fates in sea urchin embryos by overexpressing spotx, an orthodenticle-related protein  (The Company of Biologists)
90
The Company of Biologists altering cell fates in sea urchin embryos by overexpressing spotx, an orthodenticle-related protein
Screening for ATP-dependent uptake of E2-17β-G and 25OHD3-G in plasma membrane vesicles overexpressing efflux transporters. (A) E2-17β-G, a positive control substrate, was incubated at 50 μM with plasma membrane vesicles overexpressing MRP2, MRP3, BCRP, and MRP4 as well as their corresponding mock control membranes at 37°C for 5 minutes. (B) 25OHD3-G was incubated at 2 μM with plasma membrane vesicles overexpressing MRP2, MRP3, BCRP, and MRP4 as well as their corresponding mock control membranes at 37°C for 5 minutes. Data shown are means ± S.D. of triplicate determinations in a single experiment. Differences between the ATP and AMP groups and between the transporter-overexpression and the mock <t>[Sf9</t> or human embryonic kidney (HEK)] groups for each transporter were analyzed with Student’s t test; differences with P values of <0.05 were considered statistically significant. *P < 0.05; **P < 0.01; ***P < 0.001.
Altering Cell Fates In Sea Urchin Embryos By Overexpressing Spotx, An Orthodenticle Related Protein, supplied by The Company of Biologists, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/altering cell fates in sea urchin embryos by overexpressing spotx, an orthodenticle-related protein/product/The Company of Biologists
Average 90 stars, based on 1 article reviews
altering cell fates in sea urchin embryos by overexpressing spotx, an orthodenticle-related protein - by Bioz Stars, 2026-03
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chinese hamster ovary k1 cells engineered to overexpress a gr-prolink fusion protein  (DiscoverX corporation)
90
DiscoverX corporation chinese hamster ovary k1 cells engineered to overexpress a gr-prolink fusion protein
Screening for ATP-dependent uptake of E2-17β-G and 25OHD3-G in plasma membrane vesicles overexpressing efflux transporters. (A) E2-17β-G, a positive control substrate, was incubated at 50 μM with plasma membrane vesicles overexpressing MRP2, MRP3, BCRP, and MRP4 as well as their corresponding mock control membranes at 37°C for 5 minutes. (B) 25OHD3-G was incubated at 2 μM with plasma membrane vesicles overexpressing MRP2, MRP3, BCRP, and MRP4 as well as their corresponding mock control membranes at 37°C for 5 minutes. Data shown are means ± S.D. of triplicate determinations in a single experiment. Differences between the ATP and AMP groups and between the transporter-overexpression and the mock <t>[Sf9</t> or human embryonic kidney (HEK)] groups for each transporter were analyzed with Student’s t test; differences with P values of <0.05 were considered statistically significant. *P < 0.05; **P < 0.01; ***P < 0.001.
Chinese Hamster Ovary K1 Cells Engineered To Overexpress A Gr Prolink Fusion Protein, supplied by DiscoverX corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chinese hamster ovary k1 cells engineered to overexpress a gr-prolink fusion protein/product/DiscoverX corporation
Average 90 stars, based on 1 article reviews
chinese hamster ovary k1 cells engineered to overexpress a gr-prolink fusion protein - by Bioz Stars, 2026-03
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scribble planar cell polarity protein overexpression plasmid  (Shanghai GenePharma)
90
Shanghai GenePharma scribble planar cell polarity protein overexpression plasmid
Screening for ATP-dependent uptake of E2-17β-G and 25OHD3-G in plasma membrane vesicles overexpressing efflux transporters. (A) E2-17β-G, a positive control substrate, was incubated at 50 μM with plasma membrane vesicles overexpressing MRP2, MRP3, BCRP, and MRP4 as well as their corresponding mock control membranes at 37°C for 5 minutes. (B) 25OHD3-G was incubated at 2 μM with plasma membrane vesicles overexpressing MRP2, MRP3, BCRP, and MRP4 as well as their corresponding mock control membranes at 37°C for 5 minutes. Data shown are means ± S.D. of triplicate determinations in a single experiment. Differences between the ATP and AMP groups and between the transporter-overexpression and the mock <t>[Sf9</t> or human embryonic kidney (HEK)] groups for each transporter were analyzed with Student’s t test; differences with P values of <0.05 were considered statistically significant. *P < 0.05; **P < 0.01; ***P < 0.001.
Scribble Planar Cell Polarity Protein Overexpression Plasmid, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/scribble planar cell polarity protein overexpression plasmid/product/Shanghai GenePharma
Average 90 stars, based on 1 article reviews
scribble planar cell polarity protein overexpression plasmid - by Bioz Stars, 2026-03
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cho cells, which produce the fc-fusion protein and overexpress st6gal1  (Lonza)
90
Lonza cho cells, which produce the fc-fusion protein and overexpress st6gal1
Screening for ATP-dependent uptake of E2-17β-G and 25OHD3-G in plasma membrane vesicles overexpressing efflux transporters. (A) E2-17β-G, a positive control substrate, was incubated at 50 μM with plasma membrane vesicles overexpressing MRP2, MRP3, BCRP, and MRP4 as well as their corresponding mock control membranes at 37°C for 5 minutes. (B) 25OHD3-G was incubated at 2 μM with plasma membrane vesicles overexpressing MRP2, MRP3, BCRP, and MRP4 as well as their corresponding mock control membranes at 37°C for 5 minutes. Data shown are means ± S.D. of triplicate determinations in a single experiment. Differences between the ATP and AMP groups and between the transporter-overexpression and the mock <t>[Sf9</t> or human embryonic kidney (HEK)] groups for each transporter were analyzed with Student’s t test; differences with P values of <0.05 were considered statistically significant. *P < 0.05; **P < 0.01; ***P < 0.001.
Cho Cells, Which Produce The Fc Fusion Protein And Overexpress St6gal1, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cho cells, which produce the fc-fusion protein and overexpress st6gal1/product/Lonza
Average 90 stars, based on 1 article reviews
cho cells, which produce the fc-fusion protein and overexpress st6gal1 - by Bioz Stars, 2026-03
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antigen standards verify antigen standard is a collection of tagged overexpression cell lysates for use as protein immunoblot standards.  (G Biosciences)
90
G Biosciences antigen standards verify antigen standard is a collection of tagged overexpression cell lysates for use as protein immunoblot standards.
Screening for ATP-dependent uptake of E2-17β-G and 25OHD3-G in plasma membrane vesicles overexpressing efflux transporters. (A) E2-17β-G, a positive control substrate, was incubated at 50 μM with plasma membrane vesicles overexpressing MRP2, MRP3, BCRP, and MRP4 as well as their corresponding mock control membranes at 37°C for 5 minutes. (B) 25OHD3-G was incubated at 2 μM with plasma membrane vesicles overexpressing MRP2, MRP3, BCRP, and MRP4 as well as their corresponding mock control membranes at 37°C for 5 minutes. Data shown are means ± S.D. of triplicate determinations in a single experiment. Differences between the ATP and AMP groups and between the transporter-overexpression and the mock <t>[Sf9</t> or human embryonic kidney (HEK)] groups for each transporter were analyzed with Student’s t test; differences with P values of <0.05 were considered statistically significant. *P < 0.05; **P < 0.01; ***P < 0.001.
Antigen Standards Verify Antigen Standard Is A Collection Of Tagged Overexpression Cell Lysates For Use As Protein Immunoblot Standards., supplied by G Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antigen standards verify antigen standard is a collection of tagged overexpression cell lysates for use as protein immunoblot standards./product/G Biosciences
Average 90 stars, based on 1 article reviews
antigen standards verify antigen standard is a collection of tagged overexpression cell lysates for use as protein immunoblot standards. - by Bioz Stars, 2026-03
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Image Search Results


Screening for ATP-dependent uptake of E2-17β-G and 25OHD3-G in plasma membrane vesicles overexpressing efflux transporters. (A) E2-17β-G, a positive control substrate, was incubated at 50 μM with plasma membrane vesicles overexpressing MRP2, MRP3, BCRP, and MRP4 as well as their corresponding mock control membranes at 37°C for 5 minutes. (B) 25OHD3-G was incubated at 2 μM with plasma membrane vesicles overexpressing MRP2, MRP3, BCRP, and MRP4 as well as their corresponding mock control membranes at 37°C for 5 minutes. Data shown are means ± S.D. of triplicate determinations in a single experiment. Differences between the ATP and AMP groups and between the transporter-overexpression and the mock [Sf9 or human embryonic kidney (HEK)] groups for each transporter were analyzed with Student’s t test; differences with P values of <0.05 were considered statistically significant. *P < 0.05; **P < 0.01; ***P < 0.001.

Journal: Drug Metabolism and Disposition

Article Title: Hepatic Transport of 25-Hydroxyvitamin D 3 Conjugates: A Mechanism of 25-Hydroxyvitamin D 3 Delivery to the Intestinal Tract

doi: 10.1124/dmd.117.078881

Figure Lengend Snippet: Screening for ATP-dependent uptake of E2-17β-G and 25OHD3-G in plasma membrane vesicles overexpressing efflux transporters. (A) E2-17β-G, a positive control substrate, was incubated at 50 μM with plasma membrane vesicles overexpressing MRP2, MRP3, BCRP, and MRP4 as well as their corresponding mock control membranes at 37°C for 5 minutes. (B) 25OHD3-G was incubated at 2 μM with plasma membrane vesicles overexpressing MRP2, MRP3, BCRP, and MRP4 as well as their corresponding mock control membranes at 37°C for 5 minutes. Data shown are means ± S.D. of triplicate determinations in a single experiment. Differences between the ATP and AMP groups and between the transporter-overexpression and the mock [Sf9 or human embryonic kidney (HEK)] groups for each transporter were analyzed with Student’s t test; differences with P values of <0.05 were considered statistically significant. *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: Plasma membrane vesicles, including Sf9 cells overexpressing breast cancer resistance protein (BCRP), MRP2, or MRP3; HEK293 cell line cells overexpressing MRP4; and their corresponding control membrane vesicles were purchased from SOLVO Biotechnology (Szeged, Hungary).

Techniques: Clinical Proteomics, Membrane, Positive Control, Incubation, Control, Over Expression

ATP-dependent uptake of 25OHD3-G into Sf9 insect cell plasma membrane vesicles overexpressing MRP2 and MRP3. (A) 25OHD3-G at 10 μM was incubated with MRP2-overexpressing Sf9 plasma membrane vesicles or mock control membrane vesicles in the presence or absence of 50 μM MK571 at 37°C for 5 minutes. (B) 25OHD3-G at 3 μM was incubated with MRP3-overexpressing Sf9 plasma membrane vesicles or mock control membrane vesicles in the presence or absence of 50 μM MK571 or 672 μM indomethacin at 37°C for 5 minutes. Differences between the ATP and AMP groups (net ATP-dependent uptake) were calculated and compared between the MK571 or indomethacin and vehicle (dimethyl sulfoxide) treatments for statistical significance by Student’s t test. Data shown are means ± S.D. of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001, respectively. Representative kinetic profiles of MRP2- and MRP3-mediated ATP-dependent uptake of 25OHD3-G into Sf9 plasma membrane vesicles are shown in (C) and (D), respectively. 25OHD3-G of varying concentrations was incubated with membrane vesicles in the presence of ATP or AMP at 37°C for 5 minutes. The ATP-dependent net uptake into membrane vesicles was plotted against unbound concentrations of 25OHD3-G in the incubations. Data shown are means ± S.D. of triplicate determinations in a single experiment. Unbound Km and Vmax were calculated and presented as means ± S.D. of three independent experiments each with triplicate determinations.

Journal: Drug Metabolism and Disposition

Article Title: Hepatic Transport of 25-Hydroxyvitamin D 3 Conjugates: A Mechanism of 25-Hydroxyvitamin D 3 Delivery to the Intestinal Tract

doi: 10.1124/dmd.117.078881

Figure Lengend Snippet: ATP-dependent uptake of 25OHD3-G into Sf9 insect cell plasma membrane vesicles overexpressing MRP2 and MRP3. (A) 25OHD3-G at 10 μM was incubated with MRP2-overexpressing Sf9 plasma membrane vesicles or mock control membrane vesicles in the presence or absence of 50 μM MK571 at 37°C for 5 minutes. (B) 25OHD3-G at 3 μM was incubated with MRP3-overexpressing Sf9 plasma membrane vesicles or mock control membrane vesicles in the presence or absence of 50 μM MK571 or 672 μM indomethacin at 37°C for 5 minutes. Differences between the ATP and AMP groups (net ATP-dependent uptake) were calculated and compared between the MK571 or indomethacin and vehicle (dimethyl sulfoxide) treatments for statistical significance by Student’s t test. Data shown are means ± S.D. of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001, respectively. Representative kinetic profiles of MRP2- and MRP3-mediated ATP-dependent uptake of 25OHD3-G into Sf9 plasma membrane vesicles are shown in (C) and (D), respectively. 25OHD3-G of varying concentrations was incubated with membrane vesicles in the presence of ATP or AMP at 37°C for 5 minutes. The ATP-dependent net uptake into membrane vesicles was plotted against unbound concentrations of 25OHD3-G in the incubations. Data shown are means ± S.D. of triplicate determinations in a single experiment. Unbound Km and Vmax were calculated and presented as means ± S.D. of three independent experiments each with triplicate determinations.

Article Snippet: Plasma membrane vesicles, including Sf9 cells overexpressing breast cancer resistance protein (BCRP), MRP2, or MRP3; HEK293 cell line cells overexpressing MRP4; and their corresponding control membrane vesicles were purchased from SOLVO Biotechnology (Szeged, Hungary).

Techniques: Clinical Proteomics, Membrane, Incubation, Control